Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53), Carrier-free

About this product

Boster Bio Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53) catalog # MA1104. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.

Specifications

Catalog number

MA1104-carrier-free

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. Alpha-actinin (alpha A) shares structural homology with spectrin and dystrophin.

Synonyms

Protein Actn2 ;RCG30552 ;Actn2 ;rCG_30552 ;

Gene Name

actinin, alpha 2

Gene ID

Human(88), Mouse(11472), Rat(291245)

UniProt

D3ZCV0

Host

Mouse

Reactivity

Human,Mouse,Rabbit,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Rabbit skeletal alpha-actinin.

Clonality

Monoclonal

Clone

Clone: EA-53

Tissue Specificity

ACTN2: Expressed in both skeletal and cardiac muscle.

Applications

IHC,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

ACTN2: F-actin cross-linking protein which is thought to anchoractin to a variety of intracellular structures. This is a bundlingprotein.

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Youssoufian, H.; McAfee, M.; Kwiatkowski, D. J. : Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene. Am. J. Hum. Genet. 47: 62-72, 1990.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

103834 MW

Observed Molecular Weight

103 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, rabbit, By Heat <br>Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rat, rabbit, -<br>Western blot, 0.5-1μg/ml, Human, mouse, rat, rabbit<br>

Subcellular Location

ACTN2: Cytoplasm, myofibril, sarcomere, Z line|Actn2: Cytoplasm, myofibril, sarcomere, Z line

Sequence Similarities

Belongs to the alpha-actinin family.

Protein Name

Alpha-actinin-2