Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 1A4), Carrier-free

About this product

Boster Bio Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 1A4) catalog # MA1106. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

MA1106-carrier-free

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Ueyama et al. (1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.

Synonyms

Actin, aortic smooth muscle;Alpha-actin-2;Acta2;Actsa, Actvs;

Gene Name

Actin, aortic smooth muscle

Gene ID

Human(59), Mouse(11475), Rat(81633)

UniProt

P62738

Host

Mouse

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

N-terminal synthetic decapeptide of alpha-smooth muscle actin.

Clonality

Monoclonal

Clone

Clone: 1A4

Tissue Specificity

AP2A1: Isoform A expressed in forebrain, skeletalmuscle, spinal cord, cerebellum, salivary gland, heart and colon.Isoform B is widely expressed in tissues and also in breast cancerand in prostate carcinoma cells.

Applications

IF,IHC,IHC-F,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Kumar, M. S.; Hendrix, J. A.; Johnson, A. D.; Owens, G. K. : Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins. Circ. Res. 92: 840-847, 2003. 2. Ueyama, H.; Bruns, G.; Kanda, N. : Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome 10. Jpn. J. Hum. Genet. 35: 145-150, 1990.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

42009 MW

Observed Molecular Weight

42 kDa

Fragment

Mouse IgG2a

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, mouse, rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, mouse, rat, By Heat<br> Immunohistochemistry (Frozen Section), 2-5μg/ml, Human, mouse, rat, <br> Immunofluorescence, 5 μg/ml, Human, Rat<br>

Subcellular Location

Cytoplasm, cytoskeleton.

Sequence Similarities

Belongs to the adaptor complexes large subunit family.

Protein Name

Actin, aortic smooth muscle