Anti-Alpha-Tubulin TUBA1A Antibody (Monoclonal, DM1A), Carrier-free

About this product

Boster Bio Anti-Alpha-Tubulin TUBA1A Antibody (Monoclonal, DM1A) catalog # MA1107. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.

Specifications

Catalog number

MA1107-carrier-free

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Alpha-tubulin (b-alpha-1) mRNA is expressed only in brain with a molecular weight of about 55,000. The 3-prime UTR of b-alpha-1 is more than 80% homologous to the UTR of the rat brain alpha-tubulin gene, IL-alpha-T1. B-alpha-1 encodes a predicted 451-amino acid protein that is 100% identical to the rat homolog and differs by only 2 and 3 amino acids from the pig and chicken homologs, respectively.

Synonyms

Tubulin alpha-1A chain;Alpha-tubulin 3;Tubulin B-alpha-1;Tubulin alpha-3 chain;TUBA1A;TUBA3;

Gene Name

Tubulin alpha-1A chain

UniProt

Q71U36

Host

Mouse

Reactivity

Chicken,Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Microtubules from chicken embryo brain.

Clonality

Monoclonal

Clone

Clone: DM1A

Tissue Specificity

Expressed at a high level in fetal brain. .

Applications

IHC,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Cowan, N. J.; Dobner, P. R.; Fuchs, E. V.; Cleveland, D. W. : Expression of human alpha-tubulin genes: interspecies conservation of 3-prime untranslated regions. Molec. Cell. Biol. 3: 1738-1745, 1983. 2. Hall, J. L.; Cowan, N. J. : Structural features and restricted expression of a human alpha-tubulin gene. Nucleic Acids Res. 13: 207-223, 1985.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

50136 MW

Observed Molecular Weight

55 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, chicken, By Heat <br>Western blot, 1μg/ml, Human, mouse, rat, chicken<br>

Subcellular Location

Cytoplasm, cytoskeleton.

Sequence Similarities

Belongs to the adaptor complexes large subunit family.

Protein Name

Tubulin alpha-1A chain