Anti-Beta-Tubulin TUBB1 Antibody (Monoclonal, TUB 2.1), Carrier-free

About this product

Boster Bio Anti-Beta-Tubulin TUBB1 Antibody (Monoclonal, TUB 2.1) catalog # MA1112. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

MA1112-carrier-free

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Tubulin beta (TUBB) was derived from its corresponding mRNA with a molecular weight of about 55,000. TUBB gene contains 4 exons and located in the HLA class I region at 6p21.3. Paclitaxel resistance in non-small-cell lung cancer associated with beta-tubulin gene mutations.

Synonyms

Tubulin beta-1 chain;TUBB1;

Gene Name

Tubulin beta-1 chain

UniProt

Q9H4B7

Host

Mouse

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Tubulin from rat brain.

Clonality

Monoclonal

Clone

Clone: TUB 2.1

Tissue Specificity

Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins. .

Applications

IF,IHC,ICC,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). .

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Floyd-Smith, G. A.; de Martinville, B.; Francke, U. : A beta-tubulin expressed gene M40 (TUBB) is located on human chromosome 6 and two related pseudogenes are located on chromosomes 8 (TUBBP1) and 13 (TUBBP2). (Abstract) Cytogenet. Cell Genet. 40: 629, 1985. 2. Floyd-Smith, G. A.; de Martinville, B.; Francke, U. : An expressed beta-tubulin gene, TUBB, is located on the short arm of human chromosome 6 and two related sequences are dispersed on chromosomes 8 and 13. Exp. Cell Res. 163: 539-548, 1986.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

50327 MW

Observed Molecular Weight

55 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, mouse, rat, By Heat <br>Western blot, 2μg/ml, Human, mouse, rat<br>Immunocytochemistry/Immunofluorescence,5μg/ml, Human<br>

Subcellular Location

Cytoplasm, cytoskeleton .

Sequence Similarities

Belongs to the glycoprotein hormones subunit beta family.

Protein Name

Tubulin beta-1 chain