Anti-NTAL/LAT2 Antibody Picoband®, Carrier-free

About this product

Boster Bio Anti-NTAL/LAT2 Antibody catalog # PA2039. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2039-carrier-free

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

LAT2 (Linker for Activation of T Cells Family, Member 2), also known as LAB or NTAL , is a protein that in humans is encoded by the LAT2 gene. By genomic sequence analysis, Doyle et al. (2000) mapped the WBSCR5 gene to chromosome 7q11.23. They noted that the flanking genes are highly conserved between mouse and human. By immunoprecipitation and immunoblot analysis, Brdicka et al. (2002) found that NTAL associated with GRB2, SOS1, and GAB1 after B-cell receptor activation. Janssen et al. (2003) determined that phosphorylation of LAB is likely mediated by SYK upon B-cell receptor stimulation. Binding analysis indicated associations with GRB2, and, inly, with PLCG1 and PLCG2.

Synonyms

Linker for activation of T-cells family member 2;Linker for activation of B-cells;Membrane-associated adapter molecule;Non-T-cell activation linker;Williams-Beuren syndrome chromosomal region 15 protein;Williams-Beuren syndrome chromosomal region 5 protein;LAT2;LAB, NTAL, WBS15, WBSCR15, WBSCR5;HSPC046;

Gene Name

Linker for activation of T-cells family member 2

UniProt

Q9GZY6

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human NTAL.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B- cells, NK cells and monocytes. Absent from T-cells (at protein level). .

Applications

WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2mg Na2HPO4.

References & Citations

1. Brdicka, T., Imrich, M., Angelisova, P., Brdickova, N., Horvath, O., Spicka, J., Hilgert, I., Luskova, P., Draber, P., Novak, P., Engels, N., Wienands, J., Simeoni, L., Osterreicher, J., Aguado, E., Malissen, M., Schraven, B., Horejsi, V. Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J. Exp. Med. 1617-1626, 2002. 2. Doyle, J. L., DeSilva, U., Miller, W., Green, E. D. Divergent human and mouse orthologs of a novel gene (WBSCR15/Wbscr15) reside within the genomic interval commonly deleted in Williams syndrome. Cytogenet. Cell Genet. 90: 285-290, 2000. 3. Janssen, E., Zhu, M., Zhang, W., Koonpaew, S., Zhang, W. LAB: a new membrane-associated adaptor molecule in B cell activation. Nature Immun. 4: 117-123, 2003.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

26550 MW

Observed Molecular Weight

29 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Human<br>

Subcellular Location

Cell membrane ; Single-pass type III membrane protein . Present in lipid rafts.

Sequence Similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein Name

Linker for activation of T-cells family member 2