Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody, Carrier-free

Catalog number P01423-1-carrier-free

Discontinued

Price for P01423-1-carrier-free

Size / count 100 µL

This variant is discontinued and cannot be added to your cart.

About this product

Boster Bio Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody catalog # P01423-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number: P01423-1-carrier-free
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: Yes
Phospho Site: S463
Synonyms: Mothers against decapentaplegic homolog 5;MAD homolog 5;Mothers against DPP homolog 5;JV5-1;SMAD family member 5;SMAD 5;Smad5;hSmad5;SMAD5;MADH5;
Gene Name: Mothers against decapentaplegic homolog 5
UniProt: Q99717
Host: Rabbit
Reactivity: Human,Mouse,Rat
Immunogen: A synthesized peptide derived from human Phospho-Smad5 (S463/465)
Clonality: Monoclonal
Clone: Clone: AFB-19
Tissue Specificity: Ubiquitous.
Applications: WB,IHC,ICC,IF
Purification: Affinity-chromatography
Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Form: Liquid
Function: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Components: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Storage Conditions: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Calculated Molecular Weight: 52258 MW
Observed Molecular Weight: 130 kDa
Fragment: Rabbit IgG
Applications Notes: WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200
Subcellular Location: Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.

Frequently Asked Questions

What is the catalog number for Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody, Carrier-free?

The catalog number for Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody, Carrier-free is P01423-1-carrier-free.

Is Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody, Carrier-free in stock?

Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody, Carrier-free is currently made to order / not held in immediate stock. Contact us for lead time.

Where can I find the safety data sheet (SDS/MSDS) for Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody, Carrier-free?

The safety data sheet for Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody, Carrier-free is available here: https://www.bosterbio.com/msds?sku=P01423-1.

What is Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody, Carrier-free used for?

Boster Bio Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody catalog # P01423-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Is there an additional fee for hazardous or dry ice shipping of this product?

Yes, if this product requires dry ice or is classed as hazardous for transport, the additional fee will be explicitly shown in your quotation before you confirm the order, so there are no surprise charges later.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.