Anti-RIP RIPK1 Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-RIP RIPK1 Rabbit Monoclonal Antibody catalog # M00141. Tested in WB, Flow Cytometry applications. This antibody reacts with Human.

Specifications

Catalog number

M00141-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Receptor-interacting serine/threonine-protein kinase 1;2.7.11.1;Cell death protein RIP;Receptor-interacting protein 1;RIP-1;Serine/threonine-protein kinase RIP;RIPK1;RIP, RIP1;

Gene Name

Receptor-interacting serine/threonine-protein kinase 1

UniProt

Q13546

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human RIP

Clonality

Monoclonal

Clone

Clone: IGF-18

Tissue Specificity

Produced by normal alveolar macrophages and granulocytes.

Applications

WB,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

75931 MW

Observed Molecular Weight

76 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>FC 1:20

Subcellular Location

Cytoplasm. Cell membrane .

Protein Name

P2X purinoceptor 1