Anti-Bid Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-Bid Rabbit Monoclonal Antibody catalog # M00730. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Specifications

Catalog number

M00730-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

BH3-interacting domain death agonist;p22 BID;BID;BH3-interacting domain death agonist p15;p15 BID;BH3-interacting domain death agonist p13;p13 BID;BH3-interacting domain death agonist p11;p11 BID;BID;

Gene Name

BH3-interacting domain death agonist

UniProt

P55957

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Bid

Clonality

Monoclonal

Clone

Clone: AAGC-2

Tissue Specificity

Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level). .

Applications

WB,IHC,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

21995 MW

Observed Molecular Weight

90 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:1000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:10<br>FC 1:50

Subcellular Location

Cytoplasm . Mitochondrion membrane . When uncleaved, it is predominantly cytoplasmic. .

Protein Name

T-cell surface glycoprotein CD4