- Catalog number
- M03358-carrier-free
- Size / count
- 100 µL
- Dry ice
- No
- Hazardous
- No
- Discontinued
- Yes
- Synonyms
- Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1;3.1.3.86;Inositol polyphosphate-5-phosphatase of 145 kDa;SIP-145;SH2 domain-containing inositol 5'-phosphatase 1;SH2 domain-containing inositol phosphatase 1;SHIP-1;p150Ship;hp51CN;INPP5D;SHIP, SHIP1;
- Gene Name
- Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
- UniProt
- Q92835
- Host
- Rabbit
- Reactivity
- Human
- Immunogen
- A synthesized peptide derived from human SHIP
- Clonality
- Monoclonal
- Clone
- Clone: AHG-9
- Tissue Specificity
- Specifically expressed in immune and hematopoietic cells. Expressed in bone marrow and blood cells. Levels vary considerably within this compartment. Present in at least 74% of immature CD34+ cells, whereas within the more mature population of CD33+ cells, it is present in only 10% of cells. Present in the majority of T-cells, while it is present in a minority of B-cells (at protein level). .
- Applications
- WB,IHC,IP,Flow Cytometry
- Purification
- Affinity-chromatography
- Concentration
- Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Form
- Liquid
- Reconstitution
- Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
- Function
- Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol- 3,4,5-trisphosphate (PtdIns (3,4,5)P3) to produce PtdIns (3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns (1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6. .
- Components
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
- Storage Conditions
- Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 133292 MW
- Observed Molecular Weight
- 87 kDa
- Fragment
- Rabbit IgG
- Applications Notes
- WB 1:500-1:2000<br>IHC 1:50-1:200<br>IP 1:50<br>FC 1:50
- Subcellular Location
- Cytoplasm . Cell membrane ; Peripheral membrane protein . Membrane raft . Cytoplasm, cytoskeleton . Membrane ; Peripheral membrane protein . Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Translocates from the cytoplasm to membrane ruffles in a FCGR3/CD16-dependent manner. Colocalizes with FC-gamma-RIIB receptor (FCGR2B) or FCGR3/CD16 at membrane ruffles. Tyrosine phosphorylation may also participate in membrane localization. .
- Protein Name
- T-cell surface glycoprotein CD3 zeta chain
