Anti-ERK5 Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-ERK5 Rabbit Monoclonal Antibody catalog # M02812. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M02812-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Mitogen-activated protein kinase 7;MAP kinase 7;MAPK 7;2.7.11.24;Big MAP kinase 1;BMK-1;Extracellular signal-regulated kinase 5;ERK-5;MAPK7;BMK1, ERK5, PRKM7;

Gene Name

Mitogen-activated protein kinase 7

UniProt

Q13164

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human ERK5

Clonality

Monoclonal

Clone

Clone: HCD-13

Tissue Specificity

Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver. .

Applications

WB,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras- independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

88386 MW

Observed Molecular Weight

120 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:2000<br>ICC/IF 1:50-1:200<br>IP 1:50<br>FC 1:50

Subcellular Location

Cytoplasm. Nucleus. Nucleus, PML body. Translocates to the nucleus upon activation.

Protein Name

T-cell surface glycoprotein CD3 zeta chain