Anti-NEFH/Nf H Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-NEFH/Nf H Rabbit Monoclonal Antibody catalog # M05307-2. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M05307-2-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Neurofilament heavy polypeptide;NF-H;200 kDa neurofilament protein;Neurofilament triplet H protein;NEFH;KIAA0845, NFH;

Gene Name

Neurofilament heavy polypeptide

UniProt

P12036

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human NEFH

Clonality

Monoclonal

Clone

Clone: AOEO-14

Tissue Specificity

Most strongly expressed in skeletal and cardiac muscle. Also detected in smooth muscle. Weak expression in brain and lung.

Applications

WB,IHC

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

112479 MW

Observed Molecular Weight

65 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:100-1:500

Subcellular Location

Cell membrane, sarcolemma; Single-pass type II membrane protein. Cytoplasm, cytoskeleton.

Protein Name

T-cell surface glycoprotein CD3 zeta chain