Anti-HDAC1 Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-HDAC1 Rabbit Monoclonal Antibody catalog # M00256. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00256-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Histone deacetylase 1;HD1;3.5.1.98;HDAC1;RPD3L1;

Gene Name

Histone deacetylase 1

UniProt

Q13547

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human HDAC1

Clonality

Monoclonal

Clone

Clone: AIB-8

Tissue Specificity

Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.

Applications

WB,IHC,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST- mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

55103 MW

Observed Molecular Weight

34 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200

Subcellular Location

Nucleus.

Protein Name

P2X purinoceptor 1