Anti-APAF1 Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-APAF1 Rabbit Monoclonal Antibody catalog # M00889. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

M00889-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Apoptotic protease-activating factor 1;APAF-1;APAF1;KIAA0413;

Gene Name

Apoptotic protease-activating factor 1

UniProt

O14727

Host

Rabbit

Reactivity

Human,Mouse

Immunogen

A synthesized peptide derived from human APAF1

Clonality

Monoclonal

Clone

Clone: BHF-1

Tissue Specificity

Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.

Applications

WB,IHC,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

141840 MW

Observed Molecular Weight

52 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200

Subcellular Location

Cytoplasm .

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)