Anti-Wnt5a Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-Wnt5a Rabbit Monoclonal Antibody catalog # M00549. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Specifications

Catalog number

M00549-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Protein Wnt-5a;WNT5A;

Gene Name

Protein Wnt-5a

UniProt

P41221

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Wnt5a

Clonality

Monoclonal

Clone

Clone: ABEE-23

Tissue Specificity

Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level). .

Applications

WB,ICC,IF,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

42339 MW

Observed Molecular Weight

42 kDa, 48 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:1000<br>ICC/IF 1:50-1:200<br>FC 1:20

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Protein Name

T-cell surface glycoprotein CD4