Anti-Dnmt3a Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-Dnmt3a Rabbit Monoclonal Antibody catalog # M00136. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00136-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

DNA (cytosine-5)-methyltransferase 3A;Dnmt3a;2.1.1.37;DNA methyltransferase HsaIIIA;DNA MTase HsaIIIA;M.HsaIIIA;DNMT3A;

Gene Name

DNA (cytosine-5)-methyltransferase 3A

UniProt

Q9Y6K1

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Dnmt3a

Clonality

Monoclonal

Clone

Clone: EOI-4

Tissue Specificity

Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. .

Applications

WB,IHC,ICC,IF,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

101858 MW

Observed Molecular Weight

25 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:1000-1:2000<br>ICC/IF 1:100-1:500<br>FC 1:50

Subcellular Location

Nucleus . Cytoplasm . Accumulates in the major satellite repeats at pericentric heterochromatin. .

Protein Name

P2X purinoceptor 1