Anti-IKK beta IKBKB Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-IKK beta IKBKB Rabbit Monoclonal Antibody catalog # M00118. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00118-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Inhibitor of nuclear factor kappa-B kinase subunit beta;I-kappa-B-kinase beta;IKK-B;IKK-beta;IkBKB;2.7.11.10;I-kappa-B kinase 2;IKK2;Nuclear factor NF-kappa-B inhibitor kinase beta;NFKBIKB;IKBKB;IKKB;

Gene Name

Inhibitor of nuclear factor kappa-B kinase subunit beta

UniProt

O14920

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human IKK beta

Clonality

Monoclonal

Clone

Clone: AOOA-9

Tissue Specificity

Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.

Applications

WB,IHC,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Serine kinase that plays an essential role in the NF- kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

86564 MW

Observed Molecular Weight

87 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200

Subcellular Location

Cytoplasm. Nucleus. Membrane raft. Colocalized with DPP4 in membrane rafts.

Protein Name

P2X purinoceptor 1