Anti-IKK gamma IKBKG Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-IKK gamma IKBKG Rabbit Monoclonal Antibody catalog # M00874. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00874-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

NF-kappa-B essential modulator;NEMO;FIP-3;IkB kinase-associated protein 1;IKKAP1;Inhibitor of nuclear factor kappa-B kinase subunit gamma;I-kappa-B kinase subunit gamma;IKK-gamma;IKKG;IkB kinase subunit gamma;NF-kappa-B essential modifier;IKBKG;FIP3, NEMO;

Gene Name

NF-kappa-B essential modulator

UniProt

Q9Y6K9

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human IKK gamma

Clonality

Monoclonal

Clone

Clone: ABCA-9

Tissue Specificity

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Applications

WB,IHC,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'- linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B- mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

48198 MW

Observed Molecular Weight

200 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:50<br>FC 1:50

Subcellular Location

Cytoplasm . Nucleus . Sumoylated NEMO accumulates in the nucleus in response to genotoxic stress.

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)