Anti-beta Actin ACTB Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-beta Actin ACTB Rabbit Monoclonal Antibody catalog # M01263. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M01263-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Actin, cytoplasmic 1;Beta-actin;Actin, cytoplasmic 1, N-terminally processed;ACTB;

Gene Name

Actin, cytoplasmic 1

UniProt

P60709

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human beta Actin

Clonality

Monoclonal

Clone

Clone: BF-1

Tissue Specificity

Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth.

Applications

WB,IHC,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

41737 MW

Observed Molecular Weight

42 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:5000-1:50000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200

Subcellular Location

Cytoplasm, cytoskeleton . Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)