- Catalog number
- M00001-3-carrier-free
- Size / count
- 100 µL
- Dry ice
- No
- Hazardous
- No
- Discontinued
- Yes
- Synonyms
- Cellular tumor antigen p53;Antigen NY-CO-13;Phosphoprotein p53;Tumor suppressor p53;TP53;P53;
- Gene Name
- Cellular tumor antigen p53
- UniProt
- P04637
- Host
- Rabbit
- Reactivity
- Human
- Immunogen
- A synthesized peptide derived from human Mutant p53
- Clonality
- Monoclonal
- Clone
- Clone: AOA-20
- Tissue Specificity
- Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine. .
- Applications
- WB,ICC,IF,IP,Flow Cytometry
- Purification
- Affinity-chromatography
- Concentration
- Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Form
- Liquid
- Reconstitution
- Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
- Function
- Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA- Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1- mediated transcriptional activation of PER2 (PubMed:24051492). .
- Components
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
- Storage Conditions
- Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 43653 MW
- Observed Molecular Weight
- 68 kDa
- Fragment
- Rabbit IgG
- Applications Notes
- WB 1:500-1:2000<br>ICC/IF 1:50-1:200<br>IP 1:50<br>FC 1:50
- Subcellular Location
- Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Mitochondrion matrix. Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2. Translocates to mitochondria upon oxidative stress.
- Protein Name
- P2X purinoceptor 1
