Anti-Cdc27/APC3 Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-Cdc27/APC3 Rabbit Monoclonal Antibody catalog # M03905. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M03905-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Cell division cycle protein 27 homolog;Anaphase-promoting complex subunit 3;APC3;CDC27 homolog;CDC27Hs;H-NUC;CDC27;ANAPC3, D0S1430E, D17S978E;

Gene Name

Cell division cycle protein 27 homolog

UniProt

P30260

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Cdc27/APC3

Clonality

Monoclonal

Clone

Clone: IOB-3

Tissue Specificity

Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement. .

Applications

WB

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

91867 MW

Observed Molecular Weight

35 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000

Subcellular Location

Nucleus.

Protein Name

T-cell surface glycoprotein CD3 zeta chain