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Product details

Anti-CD3 epsilon Rabbit Monoclonal Antibody, Carrier-free

Catalog number M02675-2-carrier-free

Discontinued

Price for M02675-2-carrier-free

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Size / count 100 µL

This variant is discontinued and cannot be added to your cart.

About this product

Boster Bio Anti-CD3 epsilon Rabbit Monoclonal Antibody catalog # M02675-2. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human.

Specifications

Catalog number
M02675-2-carrier-free
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
Yes
Synonyms
T-cell surface glycoprotein CD3 epsilon chain;T-cell surface antigen T3/Leu-4 epsilon chain;CD3e;CD3E;T3E;
Gene Name
T-cell surface glycoprotein CD3 epsilon chain
UniProt
P07766
Host
Rabbit
Reactivity
Human
Immunogen
A synthesized peptide derived from human CD3 epsilon
Clonality
Monoclonal
Clone
Clone: EED-3
Tissue Specificity
Expressed in monocytes, basophils, B-cells, umbilical vein endothelial cells (HUVEC) and B-lymphoblastoid cells. Lower expression detected in CD4+ T-lymphocytes and natural killer cells. In the brain, detected in endothelial cells and capillaries, and in mature neurons of the frontal cortex and hippocampus. Expressed in tubular formation in the kidney. Highly expressed in astroglial tumor endothelial, microglial and glioma cells. Expressed at low levels in normal CD34+ progenitor cells, but at very high levels in several myeloid malignant cell lines. Expressed in breast carcinomas but not in normal breast tissue (at protein level). .
Applications
WB,IHC,IP,Flow Cytometry
Purification
Affinity-chromatography
Concentration
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Form
Liquid
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Function
The CD3 complex mediates signal transduction.
Components
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Storage Conditions
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Calculated Molecular Weight
23147 MW
Observed Molecular Weight
250 kDa
Fragment
Rabbit IgG
Applications Notes
WB 1:500-1:2000<br>IHC 1:50-1:200<br>IP 1:30<br>FC 1:100
Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Name
T-cell surface glycoprotein CD3 zeta chain

Frequently Asked Questions

What is the catalog number for Anti-CD3 epsilon Rabbit Monoclonal Antibody, Carrier-free?

The catalog number for Anti-CD3 epsilon Rabbit Monoclonal Antibody, Carrier-free is M02675-2-carrier-free.

Is Anti-CD3 epsilon Rabbit Monoclonal Antibody, Carrier-free in stock?

Anti-CD3 epsilon Rabbit Monoclonal Antibody, Carrier-free is currently made to order / not held in immediate stock. Contact us for lead time.

Where can I find the safety data sheet (SDS/MSDS) for Anti-CD3 epsilon Rabbit Monoclonal Antibody, Carrier-free?

The safety data sheet for Anti-CD3 epsilon Rabbit Monoclonal Antibody, Carrier-free is available here: https://www.bosterbio.com/msds?sku=M02675-2.

What is Anti-CD3 epsilon Rabbit Monoclonal Antibody, Carrier-free used for?

Boster Bio Anti-CD3 epsilon Rabbit Monoclonal Antibody catalog # M02675-2. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

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