Anti-HNF-4-alpha Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-HNF-4-alpha Rabbit Monoclonal Antibody catalog # M00389. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Specifications

Catalog number

M00389-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Hepatocyte nuclear factor 4-alpha;HNF-4-alpha;Nuclear receptor subfamily 2 group A member 1;Transcription factor 14;TCF-14;Transcription factor HNF-4;HNF4A;HNF4, NR2A1, TCF14;

Gene Name

Hepatocyte nuclear factor 4-alpha

UniProt

P41235

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human HNF-4-alpha

Clonality

Monoclonal

Clone

Clone: GBA-8

Tissue Specificity

Calprotectin (S100A8/9) is predominantly expressed in myeloid cells. Except for inflammatory conditions, the expression is restricted to a specific stage of myeloid differentiation since both proteins are expressed in circulating neutrophils and monocytes but are absent in normal tissue macrophages and lymphocytes. Under chronic inflammatory conditions, such as psoriasis and malignant disorders, also expressed in the epidermis. Found in high concentrations at local sites of inflammation or in the serum of patients with inflammatory diseases such as rheumatoid, cystic fibrosis, inflammatory bowel disease, Crohn's disease, giant cell arteritis, cystic fibrosis, Sjogren's syndrome, systemic lupus erythematosus, and progressive systemic sclerosis. Involved in the formation and deposition of amyloids in the aging prostate known as corpora amylacea inclusions. Strongly up-regulated in many tumors, including gastric, esophageal, colon, pancreatic, bladder, ovarian, thyroid, breast and skin cancers. .

Applications

WB,IHC,ICC,IF,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1- antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1- alpha. May be essential for development of the liver, kidney and intestine.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

52785 MW

Observed Molecular Weight

125 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>FC 1:50

Subcellular Location

Nucleus.

Protein Name

T-cell surface glycoprotein CD4