Anti-AMPK gamma 1 Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-AMPK gamma 1 Rabbit Monoclonal Antibody catalog # M04467. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M04467-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

5'-AMP-activated protein kinase subunit gamma-1;AMPK gamma1;AMPK subunit gamma-1;AMPKg;PRKAG1;

Gene Name

5'-AMP-activated protein kinase subunit gamma-1

UniProt

P54619

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human AMPK gamma 1

Clonality

Monoclonal

Clone

Clone: GDE-16

Tissue Specificity

Leukocytes.

Applications

WB,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by inly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

37579 MW

Observed Molecular Weight

185 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:2000<br>IP 1:20<br>FC 1:20

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

T-cell surface glycoprotein CD3 zeta chain