Anti-Cytokeratin 16 KRT16 Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-Cytokeratin 16 KRT16 Rabbit Monoclonal Antibody catalog # M03393. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Specifications

Catalog number

M03393-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Keratin, type I cytoskeletal 16;Cytokeratin-16;CK-16;Keratin-16;K16;KRT16;KRT16A;

Gene Name

Keratin, type I cytoskeletal 16

UniProt

P08779

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Cytokeratin 16

Clonality

Monoclonal

Clone

Clone: EDC-11

Tissue Specificity

Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.

Applications

WB,IHC,ICC,IF,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

51268 MW

Observed Molecular Weight

50 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>FC 1:50

Subcellular Location

Endoplasmic reticulum membrane ; Single-pass type I membrane protein . Endoplasmic reticulum . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545, PubMed:17081065). The palmitoylated form preferentially localizes to the perinuclear rough ER (PubMed:22314232). .

Protein Name

T-cell surface glycoprotein CD3 zeta chain