Anti-PI 3 Kinase Class 3 PIK3C3 Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-PI 3 Kinase Class 3 PIK3C3 Rabbit Monoclonal Antibody catalog # M02356. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M02356-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Phosphatidylinositol 3-kinase catalytic subunit type 3;PI3-kinase type 3;PI3K type 3;PtdIns-3-kinase type 3;2.7.1.137;Phosphatidylinositol 3-kinase p100 subunit;Phosphoinositide-3-kinase class 3;hVps34;PIK3C3;VPS34;

Gene Name

Phosphatidylinositol 3-kinase catalytic subunit type 3

UniProt

Q8NEB9

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human PI 3 Kinase Class 3

Clonality

Monoclonal

Clone

Clone: CDO-16

Tissue Specificity

Ubiquitously expressed, with a highest expression in skeletal muscle. .

Applications

WB,IHC

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530). Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes (By similarity). .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

101549 MW

Observed Molecular Weight

100 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200

Subcellular Location

Midbody . Late endosome . Cytoplasmic vesicle, autophagosome . As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes (Probable). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity). .

Protein Name

Lysosome-associated membrane glycoprotein 2 (Lamp2)