Anti-PI 3 Kinase p85 beta PIK3R2 Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-PI 3 Kinase p85 beta PIK3R2 Rabbit Monoclonal Antibody catalog # M03363. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Rat.

Specifications

Catalog number

M03363-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Phosphatidylinositol 3-kinase regulatory subunit beta;PI3-kinase regulatory subunit beta;PI3K regulatory subunit beta;PtdIns-3-kinase regulatory subunit beta;Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta;PI3-kinase subunit p85-beta;PtdIns-3-kinase regulatory subunit p85-beta;PIK3R2;

Gene Name

Phosphatidylinositol 3-kinase regulatory subunit beta

UniProt

O00459

Host

Rabbit

Reactivity

Human,Rat

Immunogen

A synthesized peptide derived from human PI 3 Kinase p85 beta

Clonality

Monoclonal

Clone

Clone: EOH-16

Tissue Specificity

Specifically expressed in immune and hematopoietic cells. Expressed in bone marrow and blood cells. Levels vary considerably within this compartment. Present in at least 74% of immature CD34+ cells, whereas within the more mature population of CD33+ cells, it is present in only 10% of cells. Present in the majority of T-cells, while it is present in a minority of B-cells (at protein level). .

Applications

WB,IHC,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns (4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5- trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Inly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin- dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (By similarity). .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

81545 MW

Observed Molecular Weight

70 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:50<br>FC 1:50

Subcellular Location

Cytoplasm . Cell membrane ; Peripheral membrane protein . Membrane raft . Cytoplasm, cytoskeleton . Membrane ; Peripheral membrane protein . Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Translocates from the cytoplasm to membrane ruffles in a FCGR3/CD16-dependent manner. Colocalizes with FC-gamma-RIIB receptor (FCGR2B) or FCGR3/CD16 at membrane ruffles. Tyrosine phosphorylation may also participate in membrane localization. .

Protein Name

T-cell surface glycoprotein CD3 zeta chain