- Catalog number
- M01091-carrier-free
- Size / count
- 100 µL
- Dry ice
- No
- Hazardous
- No
- Discontinued
- Yes
- Synonyms
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform;PI3-kinase subunit beta;PI3K-beta;PI3Kbeta;PtdIns-3-kinase subunit beta;2.7.1.153;Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta;PtdIns-3-kinase subunit p110-beta;p110beta;PIK3CB;PIK3C1;
- Gene Name
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
- UniProt
- P42338
- Host
- Rabbit
- Reactivity
- Human
- Immunogen
- A synthesized peptide derived from human PI3 Kinase p110 beta
- Clonality
- Monoclonal
- Clone
- Clone: EIC-16
- Tissue Specificity
- Expressed ubiquitously.
- Applications
- WB,IP,Flow Cytometry
- Purification
- Affinity-chromatography
- Concentration
- Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Form
- Liquid
- Reconstitution
- Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
- Function
- Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns (4,5)P2 (Phosphatidylinositol 4,5- bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (Phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors. .
- Components
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
- Storage Conditions
- Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 122762 MW
- Observed Molecular Weight
- 55 kDa
- Fragment
- Rabbit IgG
- Applications Notes
- WB 1:500-1:2000<br>IP 1:50<br>FC 1:50
- Subcellular Location
- Cytoplasm . Nucleus . Interaction with PIK3R2 is required for nuclear localization and export.
- Protein Name
- Glycogen synthase kinase-3 beta (gsk3b)
