Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody catalog # M01072-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M01072-1-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Actin, aortic smooth muscle;Alpha-actin-2;Cell growth-inhibiting gene 46 protein;ACTA2;ACTSA, ACTVS;GIG46;

Gene Name

Actin, aortic smooth muscle

UniProt

P62736

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human alpha smooth muscle Actin

Clonality

Monoclonal

Clone

Clone: EO-1

Tissue Specificity

Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.

Applications

WB,IHC,ICC,IF,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

42009 MW

Observed Molecular Weight

42 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:10000<br>IHC 1:500-1:1000<br>ICC/IF 1:100-1:500<br>FC 1:20

Subcellular Location

Cytoplasm, cytoskeleton.

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)