Anti-ISG15 Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-ISG15 Monoclonal Antibody catalog # M00554. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Specifications

Catalog number

M00554-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

DNA mismatch repair protein Msh6 ;hMSH6 ;G/T mismatch-binding protein ;GTBP ;GTMBP ;MutS protein homolog 6 ;MutS-alpha 160 kDa subunit;p160 ;MSH6 ;GTBP;

Gene Name

DNA mismatch repair protein Msh6

UniProt

P05161

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human ISG15

Clonality

Monoclonal

Clone

Clone: ADOH-9

Tissue Specificity

Ubiquitous.

Applications

WB,IHC,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for ing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

152786 MW

Observed Molecular Weight

42 kDa, 48 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200

Subcellular Location

Nucleus . Chromosome . Associates with H3K36me3 via its PWWP domain.

Protein Name

T-cell surface glycoprotein CD4