Anti-TRIM25 Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-TRIM25 Monoclonal Antibody catalog # M03232. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M03232-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Peptidyl-prolyl cis-trans isomerase B;PPIase B;5.2.1.8;CYP-S1;Cyclophilin B;Rotamase B;S-cyclophilin;SCYLP;PPIB;CYPB;

Gene Name

Peptidyl-prolyl cis-trans isomerase B

UniProt

Q14258

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human TRIM25 Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection.

Clonality

Monoclonal

Clone

Clone: ADFG-20

Tissue Specificity

Expressed in brain in the thalamus, in fetal and adult lung and in placenta. .

Applications

WB,IHC,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

23743 MW

Observed Molecular Weight

71 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:5000<br>IHC 1:50-1:200<br>ICC/IF 1:100-1:500<br>IP 1:50<br>FC 1:60

Subcellular Location

Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Protein Name

T-cell surface glycoprotein CD3 zeta chain