Anti-SMURF 2 Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-SMURF 2 Rabbit Monoclonal Antibody catalog # M02585. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M02585-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Neurogenic locus notch homolog protein 4;Notch 4;hNotch4;Notch 4 extracellular truncation;Notch 4 intracellular domain;NOTCH4;INT3;

Gene Name

Neurogenic locus notch homolog protein 4

UniProt

Q9HAU4

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human SMURF 2 E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then ly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation.

Clonality

Monoclonal

Clone

Clone: AECF-19

Tissue Specificity

Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.

Applications

WB

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system (By similarity). .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

209622 MW

Observed Molecular Weight

41 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:1000

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Name

T-cell surface glycoprotein CD3 zeta chain