Anti-PHOX2B Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-PHOX2B Monoclonal Antibody catalog # M02221. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

M02221-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

CD276 antigen;4Ig-B7-H3;B7 homolog 3;B7-H3;Costimulatory molecule;CD276;CD276;B7H3;PSEC0249, UNQ309/PRO352;

Gene Name

CD276 antigen

UniProt

Q99453

Host

Rabbit

Reactivity

Human,Mouse

Immunogen

A synthesized peptide derived from human PHOX2B Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element.

Clonality

Monoclonal

Clone

Clone: AEFG-16

Tissue Specificity

Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta. .

Applications

WB,IHC,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

57235 MW

Observed Molecular Weight

90 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:100-1:500<br>ICC/IF 1:50-1:200

Subcellular Location

Membrane ; Single-pass type I membrane protein .

Protein Name

Lysosome-associated membrane glycoprotein 2 (Lamp2)