Anti-IP3 Receptor Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-IP3 Receptor Rabbit Monoclonal Antibody catalog # M01465-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M01465-1-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Protein disulfide-isomerase A3;5.3.4.1;58 kDa glucose-regulated protein;58 kDa microsomal protein;p58;Disulfide isomerase ER-60;Endoplasmic reticulum resident protein 57;ER protein 57;ERp57;Endoplasmic reticulum resident protein 60;ER protein 60;ERp60;PDIA3;ERP57, ERP60, GRP58;

Gene Name

Protein disulfide-isomerase A3

UniProt

Q14643

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human IP3 Receptor

Clonality

Monoclonal

Clone

Clone: 17I50

Tissue Specificity

Detected in the flagellum and head region of spermatozoa (at protein level). .

Applications

WB,IHC,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

56782 MW

Observed Molecular Weight

314 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:500-1:1000<br>IP 1:50

Subcellular Location

Endoplasmic reticulum. Endoplasmic reticulum lumen . Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)