Anti-p150 CAF1 Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-p150 CAF1 Rabbit Monoclonal Antibody catalog # M02732-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Specifications

Catalog number

M02732-1-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Heterogeneous nuclear ribonucleoproteins C1/C2;hnRNP C1/C2;HNRNPC;HNRPC;

Gene Name

Heterogeneous nuclear ribonucleoproteins C1/C2

UniProt

Q13111

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human p150 CAF1

Clonality

Monoclonal

Clone

Clone: 19C97

Tissue Specificity

Widely expressed.

Applications

WB,IHC,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6- methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non- coding RNAs (lncRNAs) via a mechanism named 'm (6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre- mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'- UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621). .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

33670 MW

Observed Molecular Weight

150 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:50<br>FC 1:500

Subcellular Location

Nucleus. Component of ribonucleosomes.

Protein Name

T-cell surface glycoprotein CD3 zeta chain