Anti-PCYT1A Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-PCYT1A Rabbit Monoclonal Antibody catalog # M04091. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Specifications

Catalog number

M04091-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Serine/threonine-protein kinase N2;2.7.11.13;PKN gamma;Protein kinase C-like 2;Protein-kinase C-related kinase 2;PKN2;PRK2, PRKCL2;

Gene Name

Serine/threonine-protein kinase N2

UniProt

P49585

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human PCYT1A

Clonality

Monoclonal

Clone

Clone: 21P82

Tissue Specificity

Ubiquitous. Expressed in numerous tumor cell lines, especially in bladder tumor cells. .

Applications

WB,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and ion of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c- fos serum response factor (SRF). Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

112035 MW

Observed Molecular Weight

42 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>ICC/IF 1:50-1:200<br>IP 1:50<br>FC 1:50

Subcellular Location

Cytoplasm . Nucleus . Membrane . Cell projection, lamellipodium . Cytoplasm, cytoskeleton . Cleavage furrow . Midbody . Cell junction . Colocalizes with PTPN13 in lamellipodia-like structures, regions of large actin turnover. Accumulates during telophase at the cleavage furrow and concentrates finally around the midbody in cytokinesis. Recruited to nascent cell-cell contacts at the apical surface of cells. In the course of viral infection, colocalizes with HCV NS5B at perinuclear region in the cytoplasm. .

Protein Name

T-cell surface glycoprotein CD3 zeta chain