Anti-Peroxiredoxin 5 Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-Peroxiredoxin 5 Rabbit Monoclonal Antibody catalog # M02891-1. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M02891-1-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Cysteine protease ATG4B;3.4.22.-;AUT-like 1 cysteine endopeptidase;Autophagin-1;Autophagy-related cysteine endopeptidase 1;Autophagy-related protein 4 homolog B;hAPG4B;ATG4B;APG4B, AUTL1, KIAA0943;

Gene Name

Cysteine protease ATG4B

UniProt

P30044

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Peroxiredoxin 5

Clonality

Monoclonal

Clone

Clone: 22P75

Tissue Specificity

Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. .

Applications

WB,IHC,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C- terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

44294 MW

Observed Molecular Weight

17 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>IP 1:50<br>FC 1:80

Subcellular Location

Cytoplasm .

Protein Name

T-cell surface glycoprotein CD3 zeta chain