Anti-RCC1 Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-RCC1 Rabbit Monoclonal Antibody catalog # M02719. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M02719-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Abelson tyrosine-protein kinase 2;2.7.10.2;Abelson murine leukemia viral oncogene homolog 2;Abelson-related gene protein;Tyrosine-protein kinase ARG;ABL2;ABLL, ARG;

Gene Name

Abelson tyrosine-protein kinase 2

UniProt

P18754

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human RCC1

Clonality

Monoclonal

Clone

Clone: 24R07

Tissue Specificity

Widely expressed.

Applications

WB,IHC,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Non-receptor tyrosine-protein kinase that plays an ABL1- overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds ly F-actin and regulates actin cytoskeletal structure through its F-actin- bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

128343 MW

Observed Molecular Weight

45 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:100-1:500<br>IP 1:50<br>FC 1:500

Subcellular Location

Cytoplasm, cytoskeleton.

Protein Name

T-cell surface glycoprotein CD3 zeta chain