Anti-EDG3 Rabbit Monoclonal Antibody, Carrier-free

About this product

Boster Bio Anti-EDG3 Rabbit Monoclonal Antibody catalog # M03755-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Specifications

Catalog number

M03755-1-carrier-free

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

5'-AMP-activated protein kinase subunit beta-1;AMPK subunit beta-1;AMPKb;PRKAB1;AMPK;

Gene Name

5'-AMP-activated protein kinase subunit beta-1

UniProt

Q99500

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human EDG3

Clonality

Monoclonal

Clone

Clone: 25S21

Tissue Specificity

Expressed in the terminally differentiated epidermis of palms and soles. .

Applications

WB,IHC,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by inly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

30382 MW

Observed Molecular Weight

42 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200

Subcellular Location

Cytoplasm, myofibril, sarcomere, Z line . Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.

Protein Name

T-cell surface glycoprotein CD3 zeta chain