Anti-Histone H3 (mutated K27 Met) Rabbit Monoclonal Antibody, Carrier-free

Catalog number M12477-14-carrier-free

Discontinued

Price for M12477-14-carrier-free

Size / count 100 µL

This variant is discontinued and cannot be added to your cart.

About this product

Boster Bio Anti-Histone H3 (mutated K27 Met) Rabbit Monoclonal Antibody catalog # M12477-14. Tested in WB, ICC/IF, IP, ChIP applications. This antibody reacts with Human.

Specifications

Catalog number: M12477-14-carrier-free
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: Yes
Synonyms: Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3/f;Histone H3/h;Histone H3/i;Histone H3/j;Histone H3/k;Histone H3/l;HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;
Gene Name: Histone H3.1
UniProt: P68431
Host: Rabbit
Reactivity: Human
Immunogen: A synthesized peptide derived from Histone H3 (mutated K27 Met)
Clonality: Monoclonal
Clone: Clone: 26H46
Tissue Specificity: Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney. .
Applications: WB,ICC,IF,IP,ChIP
Purification: Affinity-chromatography
Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Form: Liquid
Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Components: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Storage Conditions: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Calculated Molecular Weight: 15404 MW
Observed Molecular Weight: 18 kDa
Fragment: IgG
Applications Notes: WB 1:500-1:2000<br>ICC/IF 1:50-1:200<br>IP 1:50<br>ChIP 1:50
Subcellular Location: Nucleus. Chromosome.
Protein Name: T-cell surface glycoprotein CD3 zeta chain

Frequently Asked Questions

What is the catalog number for Anti-Histone H3 (mutated K27 Met) Rabbit Monoclonal Antibody, Carrier-free?

The catalog number for Anti-Histone H3 (mutated K27 Met) Rabbit Monoclonal Antibody, Carrier-free is M12477-14-carrier-free.

Is Anti-Histone H3 (mutated K27 Met) Rabbit Monoclonal Antibody, Carrier-free in stock?

Anti-Histone H3 (mutated K27 Met) Rabbit Monoclonal Antibody, Carrier-free is currently made to order / not held in immediate stock. Contact us for lead time.

Where can I find the safety data sheet (SDS/MSDS) for Anti-Histone H3 (mutated K27 Met) Rabbit Monoclonal Antibody, Carrier-free?

The safety data sheet for Anti-Histone H3 (mutated K27 Met) Rabbit Monoclonal Antibody, Carrier-free is available here: https://www.bosterbio.com/msds?sku=M12477-14.

What is Anti-Histone H3 (mutated K27 Met) Rabbit Monoclonal Antibody, Carrier-free used for?

Boster Bio Anti-Histone H3 (mutated K27 Met) Rabbit Monoclonal Antibody catalog # M12477-14. Tested in WB, ICC/IF, IP, ChIP applications. This antibody reacts with Human.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.