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Product details

Anti-Histone H3 (acetyl K27) Rabbit Monoclonal Antibody, Carrier-free

Catalog number M12477-15-carrier-free

Discontinued

Price for M12477-15-carrier-free

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Size / count 100 µL

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About this product

Boster Bio Anti-Histone H3 (acetyl K27) Rabbit Monoclonal Antibody catalog # M12477-15. Tested in WB, IHC, ICC/IF, ChIP applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number
M12477-15-carrier-free
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
Yes
Synonyms
Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3/f;Histone H3/h;Histone H3/i;Histone H3/j;Histone H3/k;Histone H3/l;HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;
Gene Name
Histone H3.1
UniProt
P68431/P84243/Q16695/Q6NXT2/Q71DI3
Host
Rabbit
Reactivity
Human,Mouse,Rat
Immunogen
A synthesized peptide derived from Histone H3 (acetyl K27)
Clonality
Monoclonal
Clone
Clone: 26H47
Tissue Specificity
Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney. .
Applications
WB,IHC,ICC,IF,ChIP
Purification
Affinity-chromatography
Concentration
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Form
Liquid
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Components
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Storage Conditions
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Calculated Molecular Weight
15 kDa
Observed Molecular Weight
17 kDa
Fragment
IgG
Applications Notes
WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>ChIP 1:50
Subcellular Location
Nucleus. Chromosome.
Protein Name
T-cell surface glycoprotein CD3 zeta chain

Frequently Asked Questions

What is the catalog number for Anti-Histone H3 (acetyl K27) Rabbit Monoclonal Antibody, Carrier-free?

The catalog number for Anti-Histone H3 (acetyl K27) Rabbit Monoclonal Antibody, Carrier-free is M12477-15-carrier-free.

Is Anti-Histone H3 (acetyl K27) Rabbit Monoclonal Antibody, Carrier-free in stock?

Anti-Histone H3 (acetyl K27) Rabbit Monoclonal Antibody, Carrier-free is currently made to order / not held in immediate stock. Contact us for lead time.

Where can I find the safety data sheet (SDS/MSDS) for Anti-Histone H3 (acetyl K27) Rabbit Monoclonal Antibody, Carrier-free?

The safety data sheet for Anti-Histone H3 (acetyl K27) Rabbit Monoclonal Antibody, Carrier-free is available here: https://www.bosterbio.com/msds?sku=M12477-15.

What is Anti-Histone H3 (acetyl K27) Rabbit Monoclonal Antibody, Carrier-free used for?

Boster Bio Anti-Histone H3 (acetyl K27) Rabbit Monoclonal Antibody catalog # M12477-15. Tested in WB, IHC, ICC/IF, ChIP applications. This antibody reacts with Human, Mouse, Rat.

Do you offer bulk or custom quantities of this product?

Yes. Ordering two or more units, or combining this with other products, usually qualifies for a discount depending on quantity. Contact our sales team with your required volume for a custom quote.

Do you provide a Certificate of Analysis (CoA) for this product?

A Certificate of Analysis is available for most products upon request. Contact our support team with the lot number to obtain the relevant CoA.

Is this product suitable for live cell imaging?

Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.

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