- Catalog number
- M05893-carrier-free
- Size / count
- 100 µL
- Dry ice
- No
- Hazardous
- No
- Discontinued
- Yes
- Synonyms
- Golgin subfamily A member 2;130 kDa cis-Golgi matrix protein ;GM130 ;GM130 autoantigen ;Golgin-95 ;GOLGA2;
- Gene Name
- Golgin subfamily A member 2
- UniProt
- Q9BTV4
- Host
- Rabbit
- Reactivity
- Human,Mouse,Rat
- Immunogen
- A synthesized peptide derived from human TMEM43
- Clonality
- Monoclonal
- Clone
- Clone: 30T30
- Tissue Specificity
- Expressed in all tissues examined.
- Applications
- WB,IHC
- Purification
- Affinity-chromatography
- Concentration
- Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Form
- Liquid
- Reconstitution
- Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
- Function
- Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin- alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401). .
- Components
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
- Storage Conditions
- Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 113086 MW
- Observed Molecular Weight
- 45 kDa
- Fragment
- IgG
- Applications Notes
- WB 1:500-1:2000<br>IHC 1:50-1:200
- Subcellular Location
- Golgi apparatus, cis-Golgi network membrane ; Peripheral membrane protein . Cytoplasm, cytoskeleton, spindle pole . Peripheral membrane protein associated with cis-Golgi stacks (By similarity). Associates with the mitotic spindle during mitosis (PubMed:26165940). .
- Protein Name
- T-cell surface glycoprotein CD3 zeta chain
