Mouse MMP-9 ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free

About this product

Mouse <a href="https://www.bosterbio.com/human-mmp-9-picokine-trade-elisa-kit-ek0465-boster.html" style="color:#EA8D28">MMP-9 ELISA</a> Kit EZ-Set&trade; (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Mmp9 in cell culture supernatants, serum and plasma ( heparin).

Specifications

Catalog number

EZ0466-carrier-free

Size / count

5 Plates/Kit

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.

Synonyms

Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB; Mmp9; Clg4b

Gene Name

matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

Gene ID

17395

UniProt

P41245

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: A20-P730

Applications

ELISA

Sample Type

cell culture supernatants, serum and plasma (heparin).

Detection Range

156 pg/ml - 10,000 pg/ml

Function

Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (By similarity). .

Components

Description Capture Antibody Detection Antibody Avidin-Biotin-Peroxidase Complex (ABC) Lyophilized Standard|

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

80535 MW

Specificity

Natural and recombinant mouse MMP-9

Anticoagulant

heparin

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Sequence Similarities

Belongs to the peptidase M10A family.

Protein Name

Matrix metalloproteinase-9