Human CXCL7 ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free

About this product

Human CXCL7 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human PPBP in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Specifications

Catalog number

EZ0729-carrier-free

Size / count

5 Plates/Kit

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Chemokine (C-X-C motif) ligand 7 (CXCL7), also called PPBP or SCYB7, is a human gene. The encoded protein, Chemokine (C-X-C motif) ligand is a small cytokine belonging to the CXC chemokine family. CXCL7 is mapped to 4q13.3. It is a protein that is released in large amounts from platelets following their activation. CXCL7 is the precursor of the 2 platelet alpha-granule proteins, platelet basic protein (PBP) and connective tissue-activating peptide III (CTAP3). It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

Synonyms

Platelet basic protein;PBP;C-X-C motif chemokine 7;Leukocyte-derived growth factor;LDGF;Macrophage-derived growth factor;MDGF;Small-inducible cytokine B7;Connective tissue-activating peptide III;CTAP-III;LA-PF4;Low-affinity platelet factor IV;TC-2;Connective tissue-activating peptide III (1-81);CTAP-III (1-81);Beta-thromboglobulin;Beta-TG;Neutrophil-activating peptide 2 (74);NAP-2 (74);Neutrophil-activating peptide 2 (73);NAP-2 (73);Neutrophil-activating peptide 2;NAP-2;TC-1;Neutrophil-activating peptide 2 (1-66);NAP-2 (1-66);Neutrophil-activating peptide 2 (1-63);NAP-2 (1-63);PPBP;CTAP3, CXCL7, SCYB7, TGB1, THBGB1;

Gene Name

Platelet basic protein

Gene ID

5473

UniProt

P02775

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A59-D128

Applications

ELISA

Sample Type

cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Function

LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2 (73), NAP-2 (74), NAP-2 (1-66), and most potent NAP-2 (1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III (1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation. .

Components

Description Capture Antibody Detection Antibody Avidin-Biotin-Peroxidase Complex (ABC) Lyophilized Standard|

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

13894 MW

Specificity

Natural and recombinant human CXCL7

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the intercrine alpha (chemokine CxC) family.

Protein Name

Platelet basic protein