Human IL-1RA ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free

Catalog number EZ0782-carrier-free

Discontinued

Price for EZ0782-carrier-free

Size / count 5 Plates/Kit

This variant is discontinued and cannot be added to your cart.

About this product

Human IL-1RA ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IL1RN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Specifications

Catalog number: EZ0782-carrier-free
Size / count: 5 Plates/Kit
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization.3 IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.
Synonyms: Interleukin-1 receptor antagonist protein; IL-1RN; IL-1ra; IRAP; ICIL-1RA; IL1 inhibitor; Anakinra; IL1RN; IL1F3; IL1RA
Gene Name: interleukin 1 receptor antagonist
Gene ID: 3557
UniProt: P18510
Reactivity: Human
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Immunogen: Expression system for standard: E.coli; Immunogen sequence: R26-E177
Tissue Specificity: The intracellular form of IL1RN is predominantly expressed in epithelial cells.
Applications: ELISA
Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Detection Range: 31.2 pg/ml - 2,000 pg/ml
Function: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
Components: Description Capture Antibody Detection Antibody Avidin-Biotin-Peroxidase Complex (ABC) Lyophilized Standard|
Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Specificity: Natural and recombinant human IL-1RA
Anticoagulant: heparin or EDTA
Subcellular Location: Isoform 1: Secreted.

Frequently Asked Questions

What is the catalog number for Human IL-1RA ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free?

The catalog number for Human IL-1RA ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free is EZ0782-carrier-free.

Is Human IL-1RA ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free in stock?

Human IL-1RA ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free is currently made to order / not held in immediate stock. Contact us for lead time.

Where can I find the safety data sheet (SDS/MSDS) for Human IL-1RA ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free?

The safety data sheet for Human IL-1RA ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free is available here: https://www.bosterbio.com/msds?sku=EZ0782.

What is Human IL-1RA ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free used for?

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.