Human Fibronectin ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free

About this product

<a href="https://www.bosterbio.com/human-fibronectin-picokine-trade-elisa-kit-ek0349-boster.html" style="color:#EA8D28">Human Fibronectin ELISA Kit</a> EZ-Set&trade; (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human FN1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Specifications

Catalog number

EZ0349-carrier-free

Size / count

5 Plates/Kit

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer. The standard used in this kit is isolated from human plasma with the molecular mass of 200-250KDa.

Synonyms

Fibronectin; FN; Cold-insoluble globulin; CIG; Anastellin; Ugl-Y1; Ugl-Y2; Ugl-Y3; FN1; FN

Gene Name

fibronectin 1

Gene ID

2335

UniProt

P02751

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: from plasma

Tissue Specificity

Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.

Applications

ELISA

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

156 pg/ml - 10,000 pg/ml

Function

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.

Components

Description Capture Antibody Detection Antibody Avidin-Biotin-Peroxidase Complex (ABC) Lyophilized Standard|

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Fibronectin beta

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Secreted, extracellular space, extracellular matrix.