Human IL-1 beta ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free

About this product

Human <a href="https://www.bosterbio.com/human-il-1-beta-picokine-trade-elisa-kit-ek0392-boster.html" style="color:#EA8D28">IL-1 beta ELISA Kit</a> EZ-Set&trade; (DIY Antibody Pairs) (<a href="https://www.bosterbio.com/services/custom-rabbit-monoclonal-antibody-development-service/antibody-pairs-development-service" style="color:#EA8D28">antibody pairs</a> and standards for assay development). Quantitate Human IL1B in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Specifications

Catalog number

EZ0392-carrier-free

Size / count

5 Plates/Kit

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Synonyms

Interleukin-1 beta; IL-1 beta; Catabolin; IL1B; IL1F2

Gene Name

IL1B

Gene ID

3553

UniProt

P01584

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A117-S269

Tissue Specificity

Expressed in activated monocytes/macrophages (at protein level).

Applications

ELISA

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Detection Range

3.9 pg/ml - 250 pg/ml

Function

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.

Components

Description Capture Antibody Detection Antibody Avidin-Biotin-Peroxidase Complex (ABC) Lyophilized Standard|

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human IL-1 beta

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Cytoplasm, cytosol.

Protein Name

Interleukin-1 beta