Human PLAT/TPA ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free

About this product

Human PLAT/TPA ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human PLAT in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Specifications

Catalog number

EZ0897-carrier-free

Size / count

5 Plates/Kit

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Plasminogen activator, tissue, also called PLAT or TPA is a serine protease. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This gene was mapped to 8p11.21. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism.

Synonyms

Tissue-type plasminogen activator; t-PA; t-plasminogen activator; tPA; Alteplase; Reteplase; Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B; PLAT

Gene Name

PLAT

Gene ID

5327

UniProt

P00750

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: M1-P562

Tissue Specificity

Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.

Applications

ELISA

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Detection Range

31.2 pg/ml - 2,000 pg/ml

Function

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a role in facilitating neuronal migration.

Components

Description Capture Antibody Detection Antibody Avidin-Biotin-Peroxidase Complex (ABC) Lyophilized Standard|

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human PLAT/TPA

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Secreted, extracellular space.

Protein Name

Tissue-type plasminogen activator