Human IL-12(p70) ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free

About this product

Human IL-12 (p70) ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IL-12 (p70) in cell culture supernatants and serum.

Specifications

Catalog number

EZ0421-carrier-free

Size / count

5 Plates/Kit

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant human IL-12 p70 with the molecular mass of 75 KDa.

Synonyms

Interleukin 12 ;Interleukin 12, P35 ;Interleukin 12A (Natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) ;Interleukin 12A (Natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35), isoform CRA_a ;IL12A ;hCG_1685939 ;

Gene Name

interleukin 12A

Gene ID

3592

UniProt

P29459

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: (I23-S328) + (R23-S219)

Applications

ELISA

Sample Type

cell culture supernatants and serum.

Detection Range

7.8 pg/ml - 500 pg/ml

Components

Description Capture Antibody Detection Antibody Avidin-Biotin-Peroxidase Complex (ABC) Lyophilized Standard|

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

28293 MW

Specificity

Natural and recombinant human IL-12(p70)

Protein Name

Interleukin-12 subunit alpha