Human IL-6 ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free

About this product

Human IL-6 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IL6 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).

Specifications

Catalog number

EZ0410-carrier-free

Size / count

5 Plates/Kit

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.

Synonyms

cDNA FLJ61423, highly similar to Interleukin-6 ;

Gene Name

interleukin 6

Gene ID

3569

UniProt

B4DVM1

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: P29-M212

Applications

ELISA

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

4.69 pg/ml-300 pg/ml

Components

Description Capture Antibody Detection Antibody Avidin-Biotin-Peroxidase Complex (ABC) Lyophilized Standard|

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

21954 MW

Specificity

Natural and recombinant human IL-6

Anticoagulant

heparin, EDTA or citrate

Protein Name

Interleukin-6