Human IL-8 ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free

About this product

Human IL-8 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CXCL8 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Specifications

Catalog number

EZ0413-carrier-free

Size / count

5 Plates/Kit

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.

Synonyms

Interleukin-8;IL-8;C-X-C motif chemokine 8;Chemokine (C-X-C motif) ligand 8;Emoctakin;Granulocyte chemotactic protein 1;GCP-1;Monocyte-derived neutrophil chemotactic factor;MDNCF;Monocyte-derived neutrophil-activating peptide;MONAP;Neutrophil-activating protein 1;NAP-1;Protein 3-10C;T-cell chemotactic factor;MDNCF-a;GCP/IL-8 protein IV;IL8/NAP1 form I;Interleukin-8; (Ala-IL-8)77;GCP/IL-8 protein II;IL-8 (1-77);IL8/NAP1 form II;MDNCF-b;IL-8 (5-77);IL-8 (6-77); (Ser-IL-8)72;GCP/IL-8 protein I;IL8/NAP1 form III;Lymphocyte-derived neutrophil-activating factor;LYNAP;MDNCF-c;Neutrophil-activating factor;NAF;IL-8 (7-77);GCP/IL-8 protein V;IL8/NAP1 form IV;IL-8 (8-77);GCP/IL-8 protein VI;IL8/NAP1 form V;IL-8 (9-77);GCP/IL-8 protein III;IL8/NAP1 form VI;CXCL8;IL8;

Gene Name

Interleukin-8

Gene ID

3576

UniProt

P10145

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S28-S99

Applications

ELISA

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

15.6 pg/ml - 1,000 pg/ml (cell culture supernates) 7.8 pg/ml - 500 pg/ml (serum, plasma)

Function

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8 (6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8 (5-77) has increased activity on neutrophil activation and IL-8 (7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8 (1-77), respectively. .

Components

Description Capture Antibody Detection Antibody Avidin-Biotin-Peroxidase Complex (ABC) Lyophilized Standard|

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

11098 MW

Specificity

Natural and recombinant human IL-8

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the intercrine alpha (chemokine CxC) family.

Protein Name

Interleukin-8